Data acquisition is an essential poeedure affecting the efficieney and accuracy of the experiment in mass spectrometry analysis. Compared with the traditional data acquisition methods, the data independent acquisition technolggy emerging in recent yearshas the advantages of high throughput,high eoverage and high aceuraey,which has hecome a powerful tool for proteomics, metabolite quantitative and omics researeh, and has important referenee value in the field of food analysis. The principles of the curent main datain dependent acquisition and recent advance in data processing were reviewed as well as the potential of non-targeted screening in food analysis was highlighted. Furthermore,the application of non-targeted screening data independent acquisition in analysis of food safety,food anthenticity and funetional active ingredients was summarized. Finally, the challenges and future trends of data independent acquisition in food analysis was discussed.