To effectively detect the latent trace evidence on porous paper, the ultraviolet fluorescence characteristics of commonly used paper are analyzed, which provides the basis for filtering the strong background fluorescence from paper. A detection device based on ultraviolet laser-induced fluorescence is developed. To effectively stimulate the fluorescence of various trace substances, a deep ultraviolet laser is used as the excitation light source. To obtain more accurate trace information, the device receives multispectral fluorescence and compares the results. Meanwhile, the standard deviation of background gray value is utilized to measure the uniformity of paper fluorescence. The trace contrast of image is used to evaluate the trace detection effect. Five kinds of latent trace on the common paper are detected. Experimental results show that the proposed method can clearly display latent fingerprints on paper, and the contrast of fingerprint ridges is inversely proportional to the standard deviation of paper background gray. At the same time, the trace information of 10% alum solution on students work paper, 2. 5% sodium glutamate solution on copy paper, 2. 5% sodium sulfonate solution on sticky note and 0. 004% copper sulfate solution on sticky note can be clearly displayed. The proposed method has the advantages of sensibility, non-destructiveness, non-contact and multispectral. By utilizing this method, clear images of various kinds of latent trace on paper can be obtained quickly, which can provide powerful help for criminal investigation.