A set of windscreen positioning and bracket bonding detection system based on machine vision is developed in the paper.The whole design scheme of the system is described, and the system of electric control part and robot control part and machine visionpart are introduced in detail.By taking advantage of the characteristics of the image background, the backlight and double side bar at the bottom of the light with the combination of optical control strategy is used.According to the characteristics of the product, an adaptive threshold image segmentation algorithm and an improved random Hough transform algorithm are proposed to realize the automobile windshield positioning and bracket adhesion detection.The experimental results show that the designed system can well reduce the error rate of artificial bonded bracket, and the defective rate is only 4%,it meets the production precision and time requirements. The bonding precision is less than 0.5 mm, intelligent manufacturing system production efficiency is 4.5 times of artificial, the production efficiency of the enterprise is greatly improved.