The vehicle honking detection system is an innovative application of traffic management department in the field of intelligent transportation in recent years. The sound source location unit can capture the illegal honking signal of vehicle and locate the vehicle honking source by microphone array technology. In this paper, the feasibility of microphone array technology applied to the vehicle honking detection system is verified by simulation of sound localization algorithm, where we clearly point out the importance of calibration of vehicle honking detection system. Then, two calibration methods of vehicle honking detection system are studied, which include anechoic chamber testing and field testing. The validation in anechoic chamber mainly measures the location accuracy of microphone array to the sound source, while, the field testing is designed to measure the accuracy rate, the capture rate and the false alarm rate under different working conditions. According to the validation method, an experiment has been done and the results show the validity of this calibration technology in the application of vehicle honking detection system.