This paper proposes an integrated enzymefree electrochemical system for the detection and analysis of glucose based on the technologies of functional modification, MEMS and wireless communication. The nickel hydroxide (Ni (OH)2) modified screen printed electrodes were used as the sensing part, and an integrated circuit was designed for capturing, conversing and amplifying the weak signals. This circuit is composed of a microcontroller, a signal generator circuit, a constant potential circuit, an I/V conversion circuit, an amplifier circuit and an AD conversion circuit. All of these were integrated into a module with the size of a common U disk. The data was transmitted wirelessly via bluetooth and the analysis results were displayed on the upper device (PC or smart phone). The experimental results of glucose detection show that the sensitivity for glucose determination is about 1112 μA/mmol/L, and the detection limit is 459 μmol/L. Through systematic experimental evaluation, it is proved that the proposed system has a good repeatability and antiinterference ability, and the electrode activity can last for more than 48 hours. The system is expected to play a greater role in home prevention and clinical diagnosis of diabetes due to its small size, low cost and portability.