Vibroseis is an important equipment in the field of geophysical exploration. Its vibration isolation performance in low frequency band is not ideal, which affects the exploration effect to a certain extent. In this paper, KZ28 vibroseis vehicle is taken as the research object, and the negative stiffness vibration isolation system is established. By reducing the stiffness, the isolation efficiency of the original vibration isolation system in low frequency band is improved. The simulation results show that the system can be applied to the vibration isolation of vibroseis vehicle. The negative stiffness system is introduced into the vibroseis vehicle model to test the vibration isolation effect. The results show that the negative stiffness vibration isolation system can improve the isolation efficiency by more than 15%. It is feasible to apply the negative stiffness vibration isolation system to the vibroseis vehicle, which lays the foundation for the followup engineering application.