Aiming at the trouble which the tractive performance of one or more motors in intercity train tractive system decreases due to uncertainties such as parameter perturbations and unknown disturbances, resulting in the reduction of total traction, a multi-motor predefined time total collaborative control method based on virtual spindle is proposed. Firstly, considering parameter perturbations and unknown disturbances, the state equations of the multi-motor of the intercity train are established based on the tracking error of the output torque of multiple motors versus a given torque. Secondly, a novel predefined time sliding mode surface is used to design a predefined time total cooperative controller based on the total cooperative consistent control algorithm; meanwhile, the virtual spindle control strategy is used to feedback the sum of multi-motor torque to the total cooperative controller to ensure that the total train traction force is constant, and at the same time, the synchronization control performance of multi-motor is improved. Finally, the proposed control algorithm is verified by comparing simulation and hardware-in-the-loop experiments with PI control and integral sliding mode control. The total output torque of the multi-motor is kept stable within a predefined time, and the output torque of the system is able to track the given torque within 0.005 s, with a tracking error of no more than 0.03%. The results show that the method enhances the synchronization control performance of a multi-motor traction system when there are parameter perturbations and unknown disturbances in the motor.