A method using a V shaped chessboard was proposed to address the dynamic pose determination problem of 2D LiDARs mounted on uniaxial turntables in scene scanning systems. From the image of the laser stripes on the calibration planes captured by a camera, the line features of the stripes were extracted. Then the laser plane equation and two-line equations of laser stripes in the camera coordinate system were computed. Using scanning data from the LiDAR, the transformation between the LiDAR and the camera coordinate systems was acquired. By controlling the turntable rotate three times or more, the functions of the turntable axis, the LiDAR center, and the LiDAR coordinate system w. r. t the rotation angle of the turntable were estimated. In the pose determination experiment, the average error between the calculated LiDAR centers and the samples is less than 1. 2 mm, the average angle errors between the calculated LiDAR coordinate axes and the samples are less than 0. 7°. In the experiment of object measurement using a 2D LiDAR mounted on a uniaxial turntable, the object size error is less than 3 mm. The experimental results showed a good accuracy of the presented method.