In order to improve the sampling accuracy and rate of the negative temperature coefficient thermistor temperature sampling circuit, in view of the large fluctuation of hardware interference, low software execution efficiency and inability to eliminate the performance differences of the negative temperature coefficient thermistor of different individuals in the traditional temperature sampling scheme, a method based on optimizing the performance of software and hardware and eliminating the overall deviation of the temperature sampling circuit is presented. The common-cause interference is eliminated by optimizing the hardware sampling circuit, combined with the software- oversampling algorithm and using the Steinhart-Hart equation to establish an accurate mathematical model, and then the temperature calibration of the temperature-sampling module is performed to further eliminate the overall deviation. The experimental results show that using this method improves the temperature sampling accuracy from ±1.624℃ to ±0.030℃, which greatly improves the temperature sampling accuracy.