In order to provide campus service and off-campus service simultaneously, and help teachers and students to understand campus layout and resource distribution macroscopically , the paper designs an universities three-dimensional positioning AR navigation system. It uses 3DsMax, Vuforia SDK, Intent, ListView and other tools to make 3D model, identify target location ‘s flat image, switch mode and other functions. The tests show that system can meet function needs, it can enhance the rendering of scene regional goals, and achieves virtual-real fusion effect. At the same time, the system’s position error are all less than 0.6m, minimum error is 0.2m, its time is 13s, the performance can also meet students and teachers’ basic needs, the user experience is good.