ZPM check valve in the pump is easily damaged in the process of work, in order to promote production safety and efficiency. On the analysis of the check valve in the ZPM pump working condition, that combined the technology of signal analysis and processing, based on the LabVIEW, with dynamic signal acquisition card, dualcore highperformance controller and PXI chassis as the foundation of hardware, the key technology of signal acquisition for ZPM oneway valve is designed. In the process of design, the modular programming idea and the muftithread processing technology are adopted, and the designs of the loop buffer and of the data storage strategy are also carried out, at the same time. In addition, it also gives the corresponding program block diagram and code. After the test, the monitoring system is compared with the vibration signal analysis instrument of LMS International Corporation. The collected signal precision meets the requirement. Thus, the system has a longterm and continuous stable operation to achieve unattended automatic monitoring.