In order to improve the openness of the experimental teaching of power frequency X-ray machine circuit, and to solve the problems of high cost, easy damage and slow update of experimental equipment, research and development of a set of power frequency X-ray machine elemental circuit experiment simulation system for teaching, it can be completed the basic teaching tasks of the power frequency X-ray machine circuit experiment course. Combined with the requirements of the experimental teaching course of the power frequency X-ray machine circuit, taking the typical elemental circuit of the power frequency X-ray machine as an example, the circuit simulation software Multisim simulation experiment circuit, the virtual instrument development software LabVIEW is used to design the experimental system interface, and the interface technology is utilized. The communication connection between the two is realized, and the construction of the experimental system of the power frequency X-ray machine elemental circuit is completed. After the design is completed, the experimental system can simulate the working process of the actual X-ray machine elemental circuit. Students can use this system to carry out X-ray machine rectifier circuit, time-limited circuit, space charge compensation circuit and capacity protection circuit on the basis of theoretical study. Experiments with rotating anode start-up, delay, and protection circuits. The test results show that the experimental system can complete the circuit parameter measurement, circuit signal test and parameter display task in the power frequency X-ray machine unit circuit experiment course. By comparing with the real elemental circuit experiment box, it is proved that the experimental results can reflect the actual X-ray machine tube electrical characteristics. The experimental system effectively increases the hands-on practice opportunities of students, and is highly scalable, which is conducive to the exploratory circuit experiment. Therefore, the virtual instrument technology is used to design and implement the power frequency X-ray machine elemental circuit experiment system. A very effective way not only solves the problem of insufficient experimental equipment, but also provides a new idea for the design and development of other medical imaging equipment circuit experimental instruments.